![]() Oxygen and temperature changes during programmed adjustment of hypoxia.Ī single 24-well plate of cell cultures was used and monitored over 48 hrs. ( F) change in chamber O 2% profile upon inclusion of ten cell culture plates. ( E) pressure profile of experiment listed in D. ( D) change in chamber O 2% with daily gas purges in the presence of cell cultures. ( C) change in chamber O 2% with a post-1 day gas purge (in the presence of cell cultures). ( B) lack of a rapid initial increase in O 2% when chamber is devoid of any cell cultures, liquid media or water. ( A) change in chamber O 2% upon a single 4 min (20 L/min) anoxic gas purge conducted in the presence of cell cultures. Representative oxygen and temperature data transmitted by the sensor under experimental conditions. Upper panel: CoolTerm quick access bar and ribbon middle panel: format of the initial data output from CoolTerm lower panel: format of the final data saved by CoolTerm with time-stamps (saved and opened as a Microsoft Notepad file). Screen-capture images of CoolTerm terminal window. The soldered PCB was fitted on the half-sized breadboard mounted alongside the Arduino board.Ī Fritzing () sketch is presented outlining the wiring connections between the microcontroller, the logic converter, and the oxygen sensor. 64–005) to solder the PCB to the 6-pin headers. We used a 30W soldering iron (RadioShack) fitted with a 15-watt soldering iron tip (cat. The logic converter is supplied as a mini printed circuit board (PCB) with a pair of 6-pin headers to facilitate mounting on a breadboard. A3, A4 and B3, B4 pins are extra, and not needed in this application). Pin designations of the 4-channel bi-directional logic converter (low-voltage pins A1, A2 and high voltage pins B1, B2 were used. ![]() Schematic of the logic converter and connections. Required electronic parts a and accessories b. 2001, ) were used in this photograph to clearly illustrate the wire harness. 30 AWG silicone-coated (orange colored) wires (cat. ![]() ![]() (B) Layout of the modular hypoxia chamber (with the oxygen sensor mounted on half-sized breadboard), the Arduino Uno microcontroller and the wire harness routed through one of the gas-flush tube ports, before assembly of the module (lid not shown). ( A) Close-up views of the Arduino Uno microcontroller board and half-sized breadboard mounted on acrylic plate. ![]()
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